modified enzyme

Ca2 + and substrate can prohibit from the activity loss of modified enzyme and improve the fluorescence intensity compared with the control of the modification enzyme .

Ca2+和底物可减少修饰酶的失活；

Objective To evaluate accuracy and specificity of the polyanion polymer / detergent ( PPD ) method and the polyethylene glycol modified enzyme / α cyclodextrin sulfate ( PEGME ) method for direct measuring high density lipoprotein cholesterol ( HDL C ) .

目的评价聚阴离子多聚物/表面活性剂（PPD）法与PEG修饰酶/硫酸α环糊精（PEGME）法直接测定高密度脂蛋白胆固醇（HDLc）的准确性和特异性。

ALP activity was measured by modified enzyme dynamical method .

酶动力学方法测定碱性磷酸酶（ALP）活性。

Isolation and culture of osteoblast by modified enzyme digestion on mouse

小鼠成骨细胞改良酶的消化分离培养

Study on Penicillin Acylase ⅱ . Modification and Characteristics of Modified Enzyme

青霉素酰化酶研究&Ⅱ.酶的修饰和修饰酶的性质

Conclusion PSCs were isolated and cultured successfully by modified enzyme digestive method .

结论改良酶消化法可用于体外分离培养PSC，PSC分离培养的成功为进一步研究PSC活化与抑制信号通路的分子机制提供了体外模型。

In serum , the half life of modified enzyme was 90 h , 45 times of native enzyme 's.

在血清中PEG化蒜酶的半衰期为90h，是蒜酶的45倍。

The isolation of rabbit lacrimal gland epithelial cells was adopted by modified enzyme digesting .

兔泪腺上皮细胞的分离：采用改进酶消化法。

The hydrolysis activity , interfacial hydrolysis activity and esterification activity of the modified enzyme were assayed .

酶活测定：对修饰酶的水解活性和有机溶剂中的酯化活性和界面催化活性进行了测定，并与未修饰酶进行了比较。

The immunogenicity of the modified enzyme was decreased to 20-30 % of that of unmodified enzyme .

并使修饰后的天冬酰胺酶的活性保持较高的水平。结果：修饰后的天冬酰胺酶的免疫原性降低为原来的20-30%；

The results showed that this affinity chromatography column had a good binding with the modified enzyme , and can purify it from reaction mixture .

研究结果表明，由于蓖麻凝集素是一种专一性的半乳糖结合蛋白，该亲和层析柱对半乳糖苷化的胰蛋白酶表现出良好的亲和性并能有效地将其从反应混合液中分离纯化出来。

This paper describes the detection results of 49 cases of acute leptospirosis by modified enzyme labelled antibody staining technique which was compared with the improved Blenden staining method and the dark-field illumination .

本文介绍了用改良免疫酶染色法，对49例急性钩端螺旋体病人进行检查的结果，并与改良Blenden染色法和暗视野镜检法作了比较。

The results showed that the interfacial hydrolysis activity at the interface of oil-water phase and esterification activity in hexane of the modified enzyme increased apparently , although the hydrolysis activity decreased little compared with the unmodified enzyme .

实验结果表明修饰酶的水解活性略有下降，但在有机溶剂中的酯化活性和在油/水界面催化活性明显提高。

Methods : The alkaline phosphatase activity level of human dental papilla cells was determined at different time when different dose of 1,25 ( OH ) 2D 3 , Insulin , EGF and TGF - β were added into culture medium through a modified enzyme dynamical method .

方法：采用改良酶动力学的方法，测定不同浓度的1，25（OH）2D3、胰岛素、EGF及TGF－β在不同时间内对体外培养人牙乳头细胞碱性磷酸酶活性的影响。

Methods OVX were used as an experimental animal model , and a modified enzyme radioassay and an estradio ( E 2 ) radioimmunoassay for the determination of tissue aromatase activity in the brain , peripheral fat and liver tissues , and blood E 2 level , respectively .

方法以去卵巢大鼠为实验动物模型，用改进的放射酶学法和雌二醇（E2）放射免疫分析，测定脑组织和外周脂肪组织、肝组织中芳香化酶的活性及血E2的水平。

Polyethyleneglycol ( PEG ) activated by tosyl chloride was used to modify penicillin acylase ( PAC ) . The stability of the PEG PAC was much better than the free PAC . The activity of the modified enzyme was 75 % of free enzyme .

采用对甲苯磺酰氯法，用聚乙二醇对青霉素酰化酶进行化学修饰，修饰酶的比活为未修饰酶的75%，稳定性比游离酶有了很大提高。

Studies on Pharmacokinetics of Modified Fibrinolytic Enzyme from Pheretima

修饰蚯蚓纤溶酶的药代动力学研究

So we modified the enzyme in agent bovine serum albumin ( BSA ) by using glutaraldehyde as a cross-linker .

本课题用戊二醛作为交联剂，用牛血清白蛋白（BSA）进行化学修饰。

Natural sweetener , cane-sugar , stevia and liquiritin modified by enzyme methods are summarized , then the drawback and the development of enzyme modification of natural sweetener is described .

综述了运用酶来改良天然甜味剂蔗糖、甜菊糖、甘草苷等以提高它们的应用性能的方法，并指出了目前该方法存在的问题及其今后的发展方向。

Mesoporous Materials Modified PDMS-Chip for Enzyme Immobilization

介孔材料在微PDMS芯片固定化酶中的应用

The results showed that the modified electrodes and enzyme biosensors exhibited excellent electrocatalysis for H2O2 and glucose . Based on this , an accurate and rapid electroanalytical method for the quantitative analysis of glucose was developed .

该修饰电极和传感器对H2O2和葡萄糖都有较好的电催化作用，据此建立对葡萄糖的定量分析进行快速检测的方法。

Isolation of cold induced genes by modified cDNA-AFLP with single enzyme digestion from Volvariella volvacea

单酶切cDNA-AFLP改良法分离草菇冷诱导基因

The modified method of collagenase enzyme digestion-density gradient centrifugation procedure yielded a preparation primarily consisting of cells nearly the same volume with ~ 95 % viability as assessed by exclusion of the vital dye trypan blue .

采用改良的胶原酶消化-密度梯度离心法获得的细胞大小一致，活性>95%；

The best gel property of the modified SPI emerged under enzyme dosage 5 U / g , pH 8.0,37 ℃, and the protein concentration 12 % . At the same time , the gel strength was increased as the time was prolonged .

改性SPI在加酶量为5U/g、pH8.0、反应温度为37℃、蛋白浓度为12%时凝胶性改善明显，随着MTGase作用时间的延长，SPI凝胶性也呈增加趋势。